利用RAPD标记和单株树大配子体构建马尾松的分子标记连锁图谱

CONSTRUCTION OF MOLECULAR LINKAGE MAP IN MASSON PINE USING RAPD MARKERS AND MEGAGAMETOPHYTES FROM A SINGLE TREE

利用300个随机的寡核苷酸引物和马尾松(Pinus massoniana Lamb.)种子的胚乳(大配子体)产生的随机扩增多态性DNA(RAPD)分子标记,进行马尾松标记连锁图谱的构建。经筛选共获得64个稳定的RAPD标记,利用多点连锁分析,其中的48个标记分属于13个不同的连锁群(连锁对),该图谱覆盖的基因组总长度约为692.5cM(centimorgan),标记间平均距离约为14.7cM,它为马尾松连锁图谱的构建提供了一个连锁框架。

A set of 300 random oligonucleotide primers was screened for random amplified polymorphic DNA (RAPD) fragments within a sample of 79 megagametophyte DNAs of a single masson pine (Pinus massoniana Lamb.) to generate RAPD markers and construct the molecular linkage map for masson pine. 64 repeatable RAPD markers segregated in a 1 present to 1 absent ratio. Through multipoint linkage analysis, 47 markers were assigned to 13 different linkage groups (pairs) . It covered a total genetic distance of over 692.5 cM (centimorgan). Trie average distance between markers was 14.7 cM. It supplied a linkage framework for a more saturated linkage map of masson pine.