水稻F1花粉不育基因S-e的初步鉴定

Preliminary Identification of F_1 Pollen Sterility Gene S-e in Oryza sativa

选用分布于水稻12条染色体上74个多态性SSR标记，对E47—1／广陆矮4号的F2分离群体进行偏态分离分析．结果表明，9个SSR标记的分离显著或极显著地偏离了预期的孟德尔比例（1：2：1）．其中，7个SSR标记基因型偏向于籼型，2个标记偏向杂合型，没有发现偏向粳型的标记．通过对第6和第12染色体上4个SSR标记基因型与F2植株花粉育性的初步分析，表明位于第12染色体上SSR标记RM19～RM453区域附近存在1个F，花粉不育基因，定名为S-e．这一结果为分子定位该基因奠定了基础．

Seventy four SSR polymorphism markers distributed on twelve chromosome in rice between a japonica line E47-1 and an indica variety Guangluai4 had been selected to detect the distorted segregation in the E47-1/GLA F2 separate population. The results showed that nine SSR markers displayed significant deviation from the expected Mendelian ratio （1：2：1 ） at 5% or 1% level. Of these, seven SSR markers tended to indica genotypes and two markers inclined to the heterozygote. The distorted segregation at the loci RM19 and RM453 on chromosome 12 was found to be correlation with the F1 pollen sterility in the F2 population. Hereby, It suggested that there was a gene for F1 pollen sterility on the region of RM19-RM453 on chromosome 12. The locus for F1 pollen sterility is named S-e. The result will be signif- icant foundation for gene ovientation.