芒果叶中芒果甙的提取及其镇痛作用的研究

The extraction of mangiferin from mango leaves and its analgesic function

目的对芒果叶中芒果甙的提取工艺及镇痛作用进行探讨。方法采用乙醇提取、乙酸乙酯等有机溶剂从芒果叶中反复萃取芒果甙,得到淡黄色物质,并对该物质进行化学定性分析和小白鼠镇痛实验(芒果甙组),设阿司匹林组和生理盐水组进行对照分析。结果该提取物中主要含有芒果甙。经对小白鼠镇痛试验,芒果甙对扭体法致小白鼠疼痛的镇痛作用显著,与阿司匹林组和生理盐水组比较差异有显著性(P<0.01或0.05);芒果甙组在分别给药后20min、30min、60min痛阈潜伏期比生理盐水组和阿司匹林组明显延长(P均<0.01)。结论芒果叶中富含芒果甙,对小白鼠具有显著的镇痛作用。

Objective To investigate the extracting procedure of mangiferin from mango leaves and its analgesia. Methods The organic solvents of ethanol and ethyl acetate were used repeatly to extract mango leaves. A kind of light yellow substance was obtained. The chemical quality analysis for this extracted substance was performed and the analgesia test in white mice （the mangiferin group） was also performed. All the results were analytically com- pared to those in aspirin and normal saline groups Results The extracts mainly contained mangiferin. After analgesia test in small white mice, mangiferin could effectively relieve rats＇ pain induced by writhing, compared to aspirin and normal saline groups, there were statistically significant differences （all P 〈 0. 01）. Conclusion The mango leaves are abundantly contained mangiferin which has anagelsia gunction in rats.