摘要
选择最常见的禽致病性大肠杆菌O2和O78菌株,分别以常用的抗菌药物氟哌酸和氯霉素诱导,培育成遗传性稳定的耐药标记弱毒菌株O2(Norr,Chls)和O78(Chlr,Nors)。
This paper reports the development process of drug-tolerant and attenuated Escherichia coli O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s) strains. With Escherichia coli O 2 and O 78 which are the most important serotypes of E.coli in poultry colibacillosis as parent strains, drug resistant and attenuated O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s ) strains of E.coli were induced by Norfloxacin and Chloramphenicol which are two antibacterial drugs most frequently used in veterinary practice. The essential characteristics of E.coli O 2 (Nor r,Chl s ) and O 78 (Chl r,Nor s) strains were examined. The results indicated that the drug-tolerance and the heredity of E.coli O 2 (Nor r,Chl s) and O 78 (Chl r,Nor s) strains were stable. This achievement might be a good beginning for further developing efficient and multivalent vaccines by using spheroplast fusion technique.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
1997年第2期89-93,共5页
Journal of South China Agricultural University
基金
广东省自然科学基金