阴离子表面活性剂与溶菌酶相互作用的相行为

Phase behaviors of the interaction between the anionic surfactants and lysozyme

研究了pH值为7时蛋白质溶菌酶与六种阴离子表面活性剂CnH2n＋1SO4Na／CnH2n＋1SO3Na（n=8、10、12）混合体系的相行为．用紫外光谱方法考察了表面活性剂分子结构（头基、链长）、盐（缓冲溶液）对混合体系相行为的影响．结果表明，同浓度缓冲溶液中和在碳链相同时，CnH2n＋SO4Na比CnH2n＋1SO3Na更易与溶菌酶结合；在头基相同时，表面活性剂与溶菌酶的结合强度随表面活性剂的链长增加而增加．盐浓度为0～50mmol／L时，混合体系中的表面活性剂更容易形成胶团；盐浓度增大到100mmol／L时，混合体系中的表面活性剂更易与蛋白质结合．

The phase behaviors of the interactions between six anionic surfactants and lysozyrne as functions of head group, hydrocarbon chain length of surfactants and salt concentration have been investigated by UV absorbance at pH= 7. Anionic surfactants of surfactants used include CnH2n＋1 SO4Na and CnH2n＋1 SO3Na（ n =8.10,12）. CnH2n＋1 SO4 and CnH2n＋1SO3 represent sodium alkyl sulfate and sodium alkyl sulfonate, respectively. The result indicates that the interaction of lysozyme with sodium alkyl sulfate is stronger than that with sodium alkyl salfonate at the same hydrocarbon chain length. While the interaction between anionic surfactants and lysozyme became stronger with the increase of the hydrocarbon chain length at the same head group. The interaction became weaker when the salt concentration ranged from 0 mmol/L to 50 mmol/L. When the salt concentration was increased to 100 mmol/L, the interaction was enhanced.