结肠癌COX-2mRNA表达与微血管密度关系的临床意义

Clinical significance of the expression of COX-2mRNA and microvessel density in colon carcinoma

目的通过观察结肠癌组织COX-2mRNA及CD34的表达,结合结肠癌组织中微血管密度(microvessel density,MVD)所见,探讨COX-2与肿瘤血管形成及病理特征的关系,为结肠癌生物治疗提供理论基础。方法选择62例结肠癌、22例结肠腺瘤和22例正常结肠黏膜标本,采用原位分子杂交法检测COX-2mRNA并用MaxVisionTM快捷免疫组化法检测CD34表达,光镜下记数MVD。结果结肠癌、结肠腺瘤组织COX-2mRNA的阳性率明显高于正常黏膜,且有统计学意义(74.19%:36.36%,P=0.001;72.73%:36.36%,P=0.015);结肠癌组平均MVD值高于腺瘤组和正常组,三组比较,有统计学意义(F=19.628,P=0.000)。在62例结肠癌组织中,高分化组COX-2mRNA阳性率高于低分化组(χ2=4.215、P=0.040);进展期癌组的MVD高于早期癌组(t=3.079,P=0.003);淋巴结有转移组MVD高于无转移组(t=3.180,P=0.002);有血管侵犯组高于无血管侵犯组(t=2.093,P=0.041);COX-2mRNA阳性组MVD高于阴性组,COX-2mRNA高表达组MVD高于低表达组,但差异均无统计学意义(P>0.05)。结论MVD记数可作为判断肿瘤预后的有效评价指标,COX-2与肿瘤细胞的增殖和凋亡密切相关,与肿瘤血管生成无直接相关性。

Objective To study expressions of COX-2mRNA and microvessel density in colon carcinoma and their relationship among angiogenesis and pathologic character. Methods Expression of COX-2mRNA was evaluated by in situ hybridization method and expression of MVD was detected immunohistoehemieally by MaxvisionTM method by CD34 antigen in 62 cases of colon carcinoma, 22 cases of colon adenoma and 22 cases normal colon tissues. Results Expressions of COX-2mRNA were detected in a significantly greater proportion of colon carcinoma and adenoma samples than that normal colon tissues （74.19% vs 36.36% P =0.001 ; 72.73% vs 36.36% P =0.015）. The expressions of MVD were detected in a significantly greater data of colon carcinoma samples than that adenoma and normal tissues of colon （P =0. 000）. In 62 cases colon carcinoma patients, the positive expression of COX-2mRNA with high differentiation group was significantly higher than that low differentiation grouP（X2 = 4. 215, P = 0. 040） ;the expressions of MVD in advanced carcinoma group were significantly greater than that in early carcinoma group（ t = 3. 079, P = 0. 003 ） ; the expressions of MVD with lymphatic metastasis group and angiogenesis infiltration group were significantly higher than that no lymphatic metastasis group and no angiogenesis infiltration group（P = 0. 002;P = 0. 041 ） ; and the MVD was higher in COX-2mRNA positive group than that in COX-2mRNA negative group, but had no statistical eorrelationship （P 〉 0.05）. Conclusions The detected of MVD may used to judge the clinical prognosis of colon carcinoma. COX-2 may play an important role in the cell proliferation and cell apoptosis, but may not directly correlate with angiogenesis in colon carcinoma.