摘要
目的用亲和层析法从尖吻蝮蛇蛇毒中分离纯化降纤酶。方法用离子交换色谱、单克隆抗体亲和色谱技术进行分离纯化。结果得到电泳纯的降纤酶,其分子量为36000。结论用单克隆抗体亲和色谱法纯化降纤酶是切实可行的。
Objective To purify defibrase from snake venom of Agkistrodon acutus by affinity chromatography. Methods Ion exchange chro- matography and monoclonal antibody affinity chromatography were used. Results The purified defibrase showed one band in SDS- PAPGE with relative molecular weight 36KD. Conclusion The method of the monoclonal antibody affinity chromatography is a practicable production procedure.
出处
《中国药业》
CAS
2008年第2期26-27,共2页
China Pharmaceuticals
关键词
蛇毒
降纤酶
亲和层析
snake venom
defibrase
affinity chromatography
