摘要
目的筛选乌蕨中总黄酮的最佳提取工艺。方法采用正交试验设计,考察乙醇浓度、溶剂倍数、提取时间对总黄酮提取率的影响。采用分光光度法测定总黄酮的含量,并与水煎法、乙醇温浸法比较。结果乌蕨的乙醇回流提取法效果最好。结论乌蕨中总黄酮提取的最佳工艺条件为加15倍量65%的乙醇,回流提取2次,每次1h。
Objective To study the optimum condition of extraction technology for general flavonoids（GF） in Stenoloma chusanum（L. ） Ching. Methods The influences of the alcohol concentration, solvent quantity and extraction time on the extraction rate of GF were investigated. The optimized condition was determined by orthogonal design. UV- spectrophotometry was used for determination. The alcohol reflux method was compared with the decoction or alcohol warm- infusion process. Results The alcohol reflux was the best method. Conclusion The best extraction condition of total flavones contents from Stenoloma chusanum（L. ）Ching is as follows： adding 15 times volume of 65% alcohol, reflux extracting for 1.0 h and extracting twice.
出处
《中国药业》
CAS
2008年第2期42-43,共2页
China Pharmaceuticals
关键词
乌蕨
总黄酮
正交设计
提取工艺
Stenoloma chusanum（L. ）Ching
general flavonoid
orthogonal design
extraction technique
