摘要
有六个处理的一个水耕法的实验,即,0%海水(控制),10%海水,25%海水,0%海水+ N ( 7.5 mmol L ? 1 NaNO3 ),10%海水+ N ( 7.5 mmol L ? 1 NaNO3 ),并且25%海水+ N ( 7.5 mmol L ? 1 NaNO3 ),被执行在生长和菊芋(向日葵 tuberosus )的生理、生物化学的特征上学习氮增加的效果在海水应力下面的幼苗。当在 25% 海水生长显著地被禁止时, 10% 海水压力处理在植物生长上有最少的效果。在在 10% 海水下面的叶子的 malondialdehyde 内容和电解质漏类似于控制的那些,但是更高显著地在 25% 海水下面强调。在叶子的超级氧化物 dismutase,过氧化物酶和过氧化氢酶的活动与增加海水集中和时间附随地增加了。脯氨酸并且在叶子和 Na+ 可溶糖, K+ ,和 CL?在射击和根的内容与海水增加的集中显著地增加了。氮增加导致了没有 N,与海水处理相比增加射击和根的新鲜、干燥的重量。氮补充显著地也在叶子提高了抗氧化剂酶的活动。到海水的 N 的增加提高了脯氨酸的内容并且在在天线部分和 H 的根的叶子和 K+ 和 total-N 可溶糖。tuberosus,而是它导致了 Na+ 和 CL 的拒绝的集中吗?在天线部分和根。氮增加改善了由改进抗氧化的酶的海水的毒性,脯氨酸积累并且可溶糖,并且在 H 改变无机的离子的分发。tuberosus。
A hydroponic experiment with six treatments, i.e., 0% seawater (control), 10% seawater, 25% seawater, 0% seawater + N (7.5 mmol L-1 NaNO3), 10% seawater + N (7.5 mmol L-1 NaO3), and 25% seawater + N (7.5 mmol L-1 NaNO3), was carried out to study the effect of nitrogen addition on the growth and physiological and biochemical characteristics of Jerusalem artichoke (Helianthus tuberosus) seedlings under seawater stress. The 10% seawater stress treatment had the least effect on plant growth while at 25% seawater growth was significantly inhibited. The malondialdehyde content and electrolyte leakage in leaves under 10% seawater were similar to those of the control, but significantly higher under the 25% seawater stress. The activities of superoxide dismutase, peroxidase and catalase in the leaves increased concomitantly with increasing seawater concentration and time. Proline and soluble-sugars in the leaves and Na^+, K^+, and Cl- contents in shoots and roots increased significantly with the concentration of seawater increasing. Nitrogen addition resulted in increasing fresh and dry weights of shoots and roots compared with seawater treatment without N. Nitrogen supplementation also significantly enhanced the activities of antioxidant enzymes in leaves. Addition of N to seawater enhanced the contents of proline and soluble-sugars in the leaves and K^+ and total-N in the aerial parts and roots of H. tuberosus, but it resulted in declined concentrations of Na^+ and Cl- in the aerial parts and roots. Nitrogen addition ameliorated the toxicity of seawater by improving the antioxidative enzymes, accumulating of proiine and soluble-sugars, and altering the distribution of inorganic ions in H. tuberosus.
基金
Project supported by the National Science and Technology Ministry (Nos.2006BAD09A08-03-01 and 2006BAD09A04-05)
the National High Technology Research and Development Program (863 Program) of China (No.2007AA091702)
the Opening Program of Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresource and Environmental Protection (JLCBE) (No.JLCBE07001).
关键词
离子分布
向日葵
丙醛
海水应力
Helianthus tuberosus, ions distributions, malondialdehyde, nitrate, seawater stress