中国成都汉族与泰国人群P-选择素基因遗传多态性的研究

Study on Genetic Polymorphisms of P-selectins in Chinese Han of Chengdu and Thai Populations

目的研究P-选择素(P-selectin)基因启动子区-2123C/G、-1969G/A、-1817T/C和第十三外显子Thr715Pro多态性在中国成都汉族与泰国人群中的分布,同时比较不同种族间P-selectin基因型及等位基因频率分布的差异。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)测定法检测120名成都汉族人和110名泰国人P-selectin基因-2123C/G、-1969G/A、-1817T/C和Thr715Pro多态性,比较两组人群4个位点的基因型和等位基因的分布频率,并结合文献与其他种族研究结果进行比较。结果P-selectin基因启动子区-2123C/G、-1969G/A、-1817T/C多态性分布在成都汉族与泰国人群中比较差异无统计学意义(P>0.05);但与其他种族人群(英国、美国)比较,3个位点的基因型及等位基因频率分布的差异具有统计学意义(P<0.001)。本次实验未检测到Thr715Pro基因多态性。结论中国成都汉族与泰国人群中存在P-selectin基因启动子区-2123C/G、-1969G/A、-1817T/C多态性,这种基因多态性分布在成都汉族与泰国人群中比较无明显差异,与其他种族人群比较则存在显著性差异。

Objective To study the gene polymorphisms of position -2123 C/G,-1969 G/A.-1817 T/C in promoter region and of Thr715Pro in exon thirteenth of P-selectin in the Chinese Han of Chengdu and Thai populations, and simultaneously to compare distributions of genotype and allelic frequencies of P-selectins among different races. Methods The genotypes and allele frequencies of the P-selectin base -2123 C/G,-1969 G/A,- 1817 T/C and amino acid Thr715Pro were detected by polymerase chain reaction -restriction fragment length polymorphism （PCR-RFLP） to 120 healthy Chinese Han of Chengdu and 110 Thai population. Results There were no significant differences in the genotype and allele distribution of -2123 C/G,- 1969 G/A,- 1817 T/C polymorphisms for the P-selectin gene between Chinese Han of Chengdu and Thai populations （P〉0. 05）, in which compared with England and American, the distribution of P-selectin genotype and allele had significantly differences among ethnics （P〈0. 001）. No polymorphism of Thr715Pro was found in this study. Conclusion In Chinese Han of Chengdu and Thai populations the polymorphisms exist at base position -2123 C/G,-1969 G/A and -1817 T/C in promoter region of P-selectin. There are no significant differences in the genotype and allele distribution of the P-selectin gene polymorphisms between Chinese Han of Chengdu and Thai populations, but significantly different distribution of P-selectin gene polymorphisms occur among ethnics.