中国成都汉族群体五个STR基因座的遗传多态性研究

Genetic Polymorphisms of Five STR Gene Loci GATA30F04,GATA23B10,D18S847,GATA83B04 and GATA167A05 Alleles in Chinese Han Population of Chengdu Area

目的获得GATA30F04、GATA23B10、D18S847、GATA83B04和GATA167A055个STR基因座在中国成都汉族群体中的基因型及等位基因频率数据,初步探讨其在遗传学研究及法医学中的应用价值和意义。方法随机抽取100名成都地区汉族群体无血缘关系个体的静脉血,EDTA抗凝,Chelex-100法提取DNA。应用PCR扩增、非变性聚丙烯酰胺凝胶电泳、银染及测序技术分析上述5个STR基因座的遗传多态性,获得5个基因座的群体遗传学数据。结果5个基因座在该群体的基因型频率分布,均符合Hardy-Weinberg平衡定律。各基因座的期望杂合度分别为:0.694,0.918,0.836,0.889和0.880;个人识别机率分别为:0.697,0.901,0.875,0.900和0.891。结论GATA23B10、D18S847、GATA83B04和GATA167A05基因座在中国成都汉族群体中具有较高的个人识别机率,在法医学应用和群体遗传学研究中有较高的价值。

Objective To get the preliminary genotype and allele frequency distributions of GATA30F04, GATA23B10,D18S847 ,GATA83B04 and GATA167A05 loci in Chinese Han population in Chengdu area, and to validate more short tandem repeat （STR） systems for forensic application. Methods The polymerase chain reaction （PCR） was used to this study. Five STRs （GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05） were amplified from DNA samples, which were extracted with Chelex-100 method from EDTAblood of 100 unrelated individuals. The PCR products were analyzed by PAG vertical electrophoresis. Results No deviations from Hardy-Weinberg equilibrium were observed. The expected heterozygosities observed were 0. 694, 0. 918, 0. 836, 0. 889 and 0. 880 for GATA30F04, GATA23B10, D18S847, GATA83B04 and GATA167A05 respectively. The discriminating powers were 0. 697,0. 901,0. 875,0. 900 and 0, 891. Conclusion The last four loci in this study are useful markers for genetics purposes in individual identification and paternity test.