相对数统计方法在大鼠骨痂影像量化资料中的应用

Application of relative number to bony callus radiographic data in rats

目的探讨骨痂影像学资料更好的数据分析方式。方法将实验大鼠70只随机分成实验组与对照组各35只,两组大鼠均造成随机一侧胫骨中上段横形骨折,髓钉固定。实验组切断胫骨骨折侧的坐骨神经,术后30天处死全部大鼠,拍双侧胫骨侧位X光片,测量患侧胫骨骨痂最宽处及相应健侧胫骨同一水平胫骨宽度。所得数据分别用患侧宽度数据/健侧宽度数据,得到一相对数。分别用宽度的绝对数数据和相对数数据进行统计分析。结果用患侧绝对数数据进行统计,用健/患侧相对数数据进行统计,两组数据差异均有显著性。结论在骨痂影像资料对比中,用相对数数据进行统计能有效地消除个体差异,使结果客观。

Objective To investigate the appropriate ways of data analysis for radiographic image of fracture healing. Methods Seventy rats were divided into two groups randomly,the experimental group and the control group. The experimental group was inflicted with transverse fracture at random side of the tibia and the sciatic nerve resection. The control group was inflicted with transverse fracture at random side of the tibia and exposed the sciatic nerve only. Animals were executed after 30 days and lateral position radiographic image of every pair tibia was taken to evaluate the size of bony callus. Use the vernier caliper to measure the biggest width of bony callus on fracture-side tibia image and homologous uninjured-side tibia at the same height on the radiographic image. Use the fracture-side data/the uninjuredside data to obtain the relative number for every pair tibia. Analyze the data through absolute number and relative number. Results There was not statistical significance between experimental group and control group using absolute numher（P = 0. 090）; however, when relative number was used, a statistical significance was noted between the two groups （P=0. 022）. Conclusion Using the relative number to analyse the bony-callus-wide data is better than using the absolute number because the former can obviate individual variation and makes the result more objective.