不同治法中药复方对H_(22)肝癌小鼠瘤质量和体质量及免疫功能的影响

Influence of traditional Chinese compound recipes with different efficacy on body weight,tumor weight and immune function in H_(22) cancer-bearing mice

目的:比较不同治法的中药复方对H22肝癌小鼠瘤质量、体质量及免疫功能等的影响。方法:选择移植性肝癌H22小鼠为模型,观察不同治法的中药复方常规水煎剂的抑瘤作用,并通过检测各组小鼠T淋巴细胞增殖功能、自然杀伤细胞(natural killer,NK)杀伤功能、T淋巴细胞表型变化及γ干扰素(interferon-γ,IFN-γ)和白细胞介素-4(interleukin-4,IL-4)含量来比较免疫功能状况。结果:以毒攻毒组小鼠瘤质量显著小于其他各组小鼠,抑瘤率达65.8%(P<0.01);其他4组抑瘤率在10.1%～17.1%之间。以毒攻毒组小鼠体质量明显下降,出现脱毛现象;扶正培本组小鼠毛发生长良好,活泼好动。以毒攻毒组小鼠T淋巴细胞转化刺激指数(stimulation index,SI)增加显著(SI=4.34,P<0.01),说明以毒攻毒组小鼠T细胞具有较强的增殖能力;其余各组小鼠SI均低于3,表明这些组的小鼠T细胞无明显增殖作用。清热解毒、活血化瘀和以毒攻毒组小鼠NK细胞百分数显著增高,分别为生理盐水组的5.05、4.07和5.17倍(P<0.01);扶正培本和化痰散结组小鼠NK细胞杀伤活性未见显著升高。IFN-γ检测结果显示,以毒攻毒组小鼠T细胞分泌IFN-γ能力显著升高(P<0.05);IL-4检测结果显示,除扶正培本组外,清热解毒、活血化瘀、化痰散结和以毒攻毒组小鼠T细胞分泌IL-4的能力也均显著升高(P<0.01)。结论:以毒攻毒法能有效抑制肿瘤细胞的生长,明显增强小鼠细胞和体液免疫功能;扶正培本法则有良好的强壮作用。

Objective. To compare the influence of traditional Chinese compound recipes （TCCRs） with different efficacy on body weight, tumor weight and immune function in H22 cancer-bearing mice. Methods： H22 cancer-bearing mice were chosen to observe the effects of TCCRs with different efficacy on tumor growth inhibition and detect the proliferation function of T lymphocytes, the activity of natural killer （NK） cells, the changes of T lymphocytes and the content of interferon-γ （IFN-γ）and interleukin-4 （IL-4）. Results： Tumor weight of H22cancer-bearing mice in Yidu Gongdu Recipe （YDGDR, a compound traditional Chinese herbal medicine using poison as an antidote for poison）-treated group was obviously lighter than that in the other TCCR-treated groups and the tumor inhibition rate in YDGDR-treated group was 65.76% （P〈0.01）. The tumor inhibition rates in other TCCR-treated groups were ranged from 10.1% to 17.1%. Body weight of mice in YDGDR-treated group was obviously decreased and depilation was observed at the same time. Pelage of mice in Fuzheng Peiben Recipe （FZPBR, a compound traditional Chinese herbal medicine for supporting the healthy energy）-treated group grew well, and behavior of the mice was active. Stimulation index （SI） of T lymphocyte transformation in YDGDR-treated group was obviously increased （SI=4.34, P% 0.01）, which showed the proliferation function of T lymphocyte was very strong. The SI of T lymphocyte transformation in the other groups was less than three, which showed the proliferation function of T lymphocytes was not significant. Compared with normal saline （NS）-treated group, percentages of NK cells in Qinre Jiedu Recipe （QRJDR, a compound traditional Chinese herbal medicine for clearing away heat and toxic sub- stances）-treated, Huxue Huayu Recipe （HXHYR, a compound traditional Chinese herbal medicine for activating blood circulation to dissipate blood stasis）-treated and YDGDR-treated groups were obviously increased and 5.05, 4.07 and 5.17 times more than the NS-treated group, respectively （P〈0.01）. The activity of NK cells wasn＇t increased in the FZPBR-treated and HXHYR-treated groups. The production of IFN-γ induced by T cells in YDGDR-treated group was obviously raised （ P%0.05）, and the production of IL-4 induced by T cells in QRJDR-treated, HXHYR-treated, Huatan Sanjie Recipe （a compound traditional Chinese herbal medicine for eliminating phlegm and resolving masses）-treated and YDGDR-treated groups was also raised obviously （P〈0.01）. Conclusion： YDGDR has a good effect of inhibiting tumor growth and can reinforce cellular and humoral immune function in tumor-bearing mice. FZPBR can strengthen the body.