Ames试验检测三硝基甲苯及其还原代谢产物的诱变性

STUDY ON MUTAGENICITY OF TNT AND ITS REDUCTIVE METABOLITES BY AMES TEST

应用Ames试验检测系统观察了三硝基甲苯（TNT）及其还原代谢产物4-羟氨基-2，6-二硝基甲苯（4－HA），4-氨基-2，6-二硝基甲苯（4－A），2-氨基-4，6-二硝基甲苯（2－A）和2，4-二氨基-6-硝基甲苯（2，4－DA）的诱变活性。实验结果显示，三硝基甲苯及其代谢产物均具有明显的致突变作用。4-A的直接诱变活性最强，而且移码型诱变性更为显著。在微粒体酶系统的参与下，TNT的诱变活性增强，其代谢产物的诱变性有增有减，4-位硝基的还原活化和4-氨基的直接诱变能力，在TNT的诱变性中起重要作用。

The mutagenicity of TNT and its metabolites 4-hydroxylamino-2, 6-dinitrotoluene (4-HA), 4-amino- 2, 6-dinitrotoluene (4-A), 2-amino-4, 6-dinitrotoluene (2-A) and 2, 4-diamino-6-nitrotoluene (2,4-DA) has been detected with Ames test. The results showed that TNT and its metabolites all have remarkable mutagenicity. The direct mutagenicity of 4-A is the highest. Indirect mutagenicity of TNT elevates significantly in the presence of S-9mix and it is higher than that of 4-A. Reductive activation of 4-nitro group and direct mutagenicity of 4-amino compounds are of importance for mutagenic activity of TNT.