加减清营汤对H_2O_2损伤的血管内皮细胞存活率和SOD活力的影响

Effects of Jiajian Qingying Recipe on the Survival Rate and the Level of SOD of VEC

目的:观察加减清营汤对H2O2损伤的血管内皮细胞存活率和SOD活力的影响。方法:体外培养内皮细胞ECV304,用380μmol/LH2O2损伤细胞,随机分成正常组、模型组、血清对照组、丹参组、加减清营汤含药血清组。用血清药理学方法给药,继续培养后应用MTT法观察细胞存活率,检测SOD。结果表明:加减清营汤含药血清组MTT的OD值在24h点高于H2O2模型组(P<0.01),与血清对照组无明显差异(P>0.05),36h后与血清对照组有明显差异(P<0.01)。加减清营汤含药血清组SOD值高于模型组、血清对照组(P<0.01)。结论:加减清营汤可降低H2O2对ECV304的损伤程度,其机理可能与抗氧化作用有关。

Objective： The study was designed to investigate the effects of Jiajian Qingying recipe on the survival rate and the level of SOD of ECV304. Methods ： To make drug - containing serum with the method of seropharm acology, to establish the cell model by H2O2,and observe the effects on the survival rate and the level of SOD. Results： The survival rate of drug - containing serum group was more than model group, and there was no significant differences between drug -containing serum group and serum contrast group until after 36h. And the level of SOD of drug - containing serum group was obviously higher than the other groups. Conclusion ： Jiajian Qingying recipe can obviously protect ECV304 from injury induced by H2O2. Its mechanism may be related to the fuction of antioxidative.