ERIC-PCR指纹图谱技术对低温下SUFR反应器中菌群结构分析

Analysis of the microbial community structure of a Spiral up-flow reactor (SUFR) at low temperature by ERIC-PCR fingerprinting

使用ERIC-PCR指纹图谱技术对螺旋升流式反应器(SpiralUp-FlowReactor,SUFR)在低温下的微生物群落结构进行了分析.结果表明,低温下反应器中微生物菌群呈多样性分布,优势菌群突出;在遭受约5℃低温条件冲击后,反应器中菌群多样性和反应器脱氮除磷效率都有所降低,但TN去除率和TP去除率仍然达到了72.35%和93.28%,维持了反应器的正常功能.不同温度下对比分析可以看出,在低温和常温下反应器中优势菌群发生了一定变化,常温条件下的优势菌群比低温条件下更丰富多样.总体看来,SUFR反应器良好的螺旋升流特质有利于不同菌群在不同温度下的功能发挥,特别是低温下脱氮除磷功能的维持.

The microbial community structure of activated sludge in a Spiral up-flow reactor（SUFR） operated under low temperature conditions was investigated by ERIC-PCR fingerprinting. The results indicated a high diversity of the bacterial community in the SUFR reactor at low temperature and some apparently dominant bacteria. At 5℃, the bacteria! diversity and the number of predominant bacteria decreased, but the removal efficiency of TN and TP still reached 72.35% and 93.28% respectively, so the SUFR reactor maintained its normal function. Comparative analysis of the bacterial community at low temperature and normal temperature showed that the SUFR reactor produced different kinds of predominant bacteria at different temperatures, and the diversity of the predominant bacteria at normal temperature was much greater than that at low temperature. Therefore, the SUFR reactor, with its spiral-up design, facilitates grows of different kinds of functional bacteria at different temperatures, which allows it to maintain good nitrogen and phosphorus removal at low temperatures.