散发性阿尔茨海默病患者血清脑源性神经营养因子的测定及临床意义

Clinical Significance of Serum Levels of Brain-derived Neurotrophic Factor in Patients of Sporadic Alzheimer Disease

目的探讨散发性阿尔茨海默病(sporadic Alzhei mer disease,SAD)患者血清脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)变化及其临床意义。方法选择2004-11—2005-10作者医院门诊及住院SAD患者43例,其中轻度痴呆18例,中度痴呆19例,重度痴呆6例。对照组35例为同期体检者。采用酶联免疫吸附法(ELISA)测定外周血清BDNF水平。结果SAD组和对照组间性别(χ2=0.0042,P=0.9483)、年龄(t=0.38,P=0.7023)及血清BDNF水平(t=0.60,P=0.5528)差异均无统计学意义,轻、中、重度SAD组及对照组间血清BDNF水平比较亦无统计学意义(F=0.89,P=0.4526)。结论SAD患者外周血清BDNF水平无明显变化,血清BDNF水平不能作为SAD诊断的标志物,也不能反映痴呆的严重程度。

Objective To investigate the clinical significance of brain-derived neurotrophic factor （BDNF） from peripheral serum in patients of sporadic Alzheimer disease （SAD）. Methods 43 out and inpatients of SAD were chosen according to DSM-IV and NINCDS-ADRDA criteria for ＇ probable AD＇ from 2004 November to 2005 October, including 18 mild dementia patients, 19 moderate dementia patients and 6 severe dementia patients. 35 controls were chosen simultaneously in health examinated people. Enzyme-linked immunosorbant assay （ELISA） was used to measure the serum levels of BDNF. Results Gender, age and the level of BDNF showed no significantly difference in AD and control group （x^2=2= 0. 0042, P=0. 9483; t=0.38, P=0. 7023; t=0.60,P= 0. 5528, respectively）. When SAD patients were divided into 3 groups, no significant differences were observed among mild dementia, moderate dementia, severe dementia and control group （F= 0.89, P = 0.4526）. Conclusions No significant differences were demonstrated in peripheral serum levels of BDNF between SAD patients and controls. Serum levels of BDNF could not be as a candidate marker for clinical diagnosis and could not reflect the severity of dementia.