ZTC1+1天然澄清剂在桑叶多糖提纯中的应用

Application of Natural Clarifying Agent of ZTC1+1 in Purifying Polysaccharides from Mulberry Leaves

考察Ⅱ型ZTC1+1天然澄清剂在桑叶多糖提取中的提纯效果。以硫酸-蒽酮法测定多糖含量,通过正交试验优化天然澄清剂处理工艺。试验表明Ⅱ型ZTC1+1天然澄清剂能有效地保留桑叶中的有效成分多糖。适宜工艺条件为药液浓缩程度为5 mL/g,澄清剂B和A两组分的加入量分别为浓缩药液体积的2%和1%,絮凝温度50℃。在此工艺下,多糖保留率为96%,浸膏中多糖含量约为22%,蛋白去除率约为16%。

This paper aims to study the effect of natural clarifying agent of Ⅱ ZTC1 ＋ 1 on the purification of polysaccharides extracted from mulberry leaves. The content of polysaccharides in the extracts was determined by anthracenone-sulphuric acid method and the optimum conditions were obtained by orthogonal experimentations.The results showed that the natural clarifying agent of Ⅱ ZTC1 ＋ 1 can effectively extract polysaccharides from mulberry leaves, while some impurities were removed. The optimum experimental conditions were as follows ： concentration ratio is 5 ： 1 mL/g; the adding amount of natural clarifying agent （i. e B and A） to the concentrated solution are 2% and 1% respectively the clarifying temperature is 50℃. Under above conditions, the retention ratio of polysaccharides was above 96 %, the content of polysaccharides in the extracts was near 22% and the removal of proteins was 16%.