锰对雄性小鼠中脑单胺类神经递质含量影响

Effects of manganese on monoamine content of midbrains in male rats

目的探讨锰对雄性小鼠中脑单胺类神经递质含量的影响。方法将90只雄性昆明系小鼠随机分为3组(30,15 mg/kg,盐水对照组),每日腹腔注射染毒,连续染6 d停染1 d。分别于染毒20,40,60 d以后,颈椎脱臼处死。迅速剥出小鼠的全脑用锡箔包好后,置于液氮中保存。然后将组织制成匀浆并冷冻离心,取上清液移入离心管中。用高效液相色谱仪的紫外检测器测定其中单胺类神经递质的含量,并同时计算小鼠脏器系数。结果3种单胺类神经递质多巴胺(DA)、去甲肾上腺素(NE)、五羟色胺(5-HT)的含量均低于盐水对照组,呈不断下降趋势。其中多巴胺的含量在40,60 d时下降明显,差异有统计学意义,P<0.05。同时发现,2个染毒组的雄性小鼠肝脏、脾脏、脑组织、以及睾丸的脏器系数与盐水对照组比较,均有不同程度变化,肝脏、脾脏的脏器系数增高,而脑组织、睾丸的脏器系数却降低。结论金属锰的长期接触可能会影响雄性小鼠中脑单胺类神经递质含量,并且对某些脏器有一定的毒性作用。

Objective To study the effects of manganese on monoamine contents of midbrains in male rats.Methods 90 Kunming male rats were divided into three groups respectively（30,15 mg/Kg,and the control group）.Male rats were given intraperitoneal-injection containing manganese chloride at the same time every six days.They were sacrificed by vertebra disjoint at every 20 days later.The whole brains were peeled off quickly,and were wrapped with silver paper.Then they were put in the liquid nitrogen.The tissues were made into plasm,and then frozen-centrifugal machine was used to distill the upper liquid.The ultravioletradiation detecting system of HPLC was used to mensurate the monoamine in the samples.While the organ relative of male rats was determined.Results Contents of the three monoamines were all decreased than the salt control group and showed a downward trend.The contents of DA in 40 days and 60 days dropped obviously and had remarkable difference after the statistical examination.Male rats＇ organ relatives in the two groups exposed to manganese were all changed a lot.The organ relatives of liver and spleen were increased,but the rest were all decreased.Conclusion The perennial contacting of manganese may affect the monoamine content of midbrains in the male rats,while also has certain toxic effect to some organs.