摘要
目的探讨乙酰肝素酶蛋白在喉癌中的表达规律以及与CerbB-2表达的关系。方法喉癌组为58例肿瘤组织保存蜡块,其中临床分期:Ⅰ期7例,Ⅱ期14例,Ⅲ期26例,Ⅳ期11例;病理分级:高分化15例,中分化22例,低分化21例。另选择19例癌旁组织作对照。采用免疫组织化学SABC染色法检测乙酰肝素酶与CerbB-2的表达情况。结果喉癌组乙酰肝素酶蛋白阳性表达率为77.6%(45/58),显著高于癌旁组织(0/19)。CerbB-2阳性率分别为癌组织70.7%(41/58)和癌旁组织15.8%(3/19)。乙酰肝素酶表达与肿瘤临床分期及淋巴结转移显著相关,CerbB-2表达还与病理分级有关。结论乙酰肝素酶在喉癌组织中高表达,联合检测乙酰肝素酶与CerbB-2有助于喉癌患者预后的判断。
Objective To explore the expression regulation of heparanase and CerbB-2 in larynx cancer of different period. Methods Fifty-eight specimens of patients sufferred from larynx cancer were included in this experiment. Among them,7cases were in clinical stage Ⅰ , 14 in stage Ⅱ ,26 in stage Ⅲ and 11 in stage Ⅳ. Another 19 latero-tissue away from cancer of larynx were included in the control group. The expression of heparanase was detected with the technology of immunohistochemistry staining. Results In the control group,the positive heparanase was 0%. In the cancer group,the positive rate of heparanase was 77.6% （45/58）, which was higher than that in the control group. The positive rate of CerbB-2 was 70. 7% （41/58） in the carcinoma group and 15.8%（3/19） in the control group respectively. According to correlation analysis,the expression of heparanase and CerbB-2 was related to clinical stage and lymph node metastasis of tumor. Conclusion The abnormal high expression of heparanase plays an important role in the occurrence of larynx cancer. The detection of heparanase and CerbB-2 is an effectively index in the diagnosis and prognosis of larynx cancer.
出处
《重庆医学》
CAS
CSCD
2008年第1期62-64,共3页
Chongqing medicine
