辐射引起人结肠癌细胞系HT-29中STAT6蛋白活化

Ionizing Radiation Induces the Phosphorylation of STAT6 in Colon Cancer Cell Line HT29

目的:研究电离辐射能否导致人结肠癌细胞系HT-29中信号转导子与转录活化子6(STAT6)蛋白活化,从而探讨肿瘤出现放射抗拒的可能机制。方法:用流式细胞仪检测HT-29细胞接受2 Gy射线照射后30 min、1h、2 h细胞内STAT6蛋白的活化情况。结果:HT-29细胞接受射线照射后出现STAT6的活化,在照射后1 h达高峰(P<0.01),2 h后稍高于照射前水平(P<0.05)。结论:射线可以引起HT-29细胞中STAT6蛋白的活化,从而导致细胞出现放射后的凋亡抑制,可能部分解释肿瘤细胞的放射抗拒性。

Objective： To investigate whether ionizing radiation can induce the phosphorylation of STAT6 （Signal Transducer and Activator of Transcription 6） ,which may shed a light on the radioresistance of HT29 cell line. Methods： Flow cytometry was used to detect the phosphorylation of STAT6 in HT29 cells at different time after radiation. Results： Radiation could induce the phosphorylation of STAT6 in HT29 cell line, which reached the peak at 1 h after radiation and was remain a higher level than pre-radiation till 2 h after radiation. Conclusion： Radioresistance of HT29 may be explained partially by the phosphorylation of STAT6 after radiation, which has been demonstrated playing an important role in inhibiting apoptosis in HT29.