壳寡糖对神经干细胞分化的影响

Effect of chitooligosaccharide on neuronal differentiation of neurospheres

目的:探讨壳聚糖的降解产物壳寡糖对神经干细胞分化的影响。方法:将壳聚糖降解为分子量1100能够溶于水的壳寡糖,然后将不同浓度的壳寡糖(0.1mg/ml,0.01mg/ml)与神经干细胞共培养12天,研究壳寡糖对神经干细胞分化的影响,对照组采用无壳寡糖培养。结果:实验组的神经干细胞分化的突起平均长度比对照组要长,表明一定浓度的壳寡糖能够促进神经干细胞的分化。结论:用壳聚糖制备人工神经移植物时,壳聚糖导管不仅在神经再生过程可起到支架,细胞依附的作用,同时其降解产物壳寡糖能够起到促进神经生长的作用,而进一步阐明壳聚糖具有多功能的生物学作用。

Objective： To investigate the effect of chitooligosaccharide on neuronal differentiation of neurospheres. Methods： Chitosan is degraded into chitooligosaccharide. Its molecular weight is 1100 and can be soluble in water. The neurospheres were digested and dissociated into single cell suspension with trypsin and then divided into three groups. Two experimental groups were cultured in the above-mentioned medium with chitooligosaccharide having the concentrations of 0.1 and 0.01mg/ml, respectively. The control group was cultured in the same manner but without chitooligosaccharide. Results： The length of neurite outgrowth of neurosphere cells in both experimental groups （0.01 and 0.1mg/ml chitooligosaccharide respectively） was longer than that in the control group after culture for 12 days. Conclusion： When chotosan was used as biomaterial to prepare artificial nerve graft, chitosan not only acts as a supporting scaf-fold to guide axonal growth, but also promote neuronal differentiation of neurospheres.