去细胞组织工程心脏瓣膜构建的实验研究

Experimented study on creating acellular tissue engineering heart valve

目的:探讨人脐静脉内皮细胞(HUVECs)种植在去细胞瓣叶上构建组织工程心脏瓣膜的可行性。方法:测定瓣叶去细胞前、后的生物力学性能;观察去细胞瓣叶上内皮细胞生长情况。结果:瓣叶去细胞可获得完整无细胞的纤维支架,瓣叶去细胞前、后的生物力学性能无差异;内皮细胞在去细胞瓣叶表面生长,形成一层基本连续的细胞层。结论:去细胞瓣叶组织是一种良好的纤维支架,可以用于构建组织工程瓣膜;内皮细胞种植在去细胞瓣叶上构建组织工程心脏瓣膜是可行的。

Objective：To investigate the feasibility of creating tissue engineering heart valve from human umbilical vein en-dostelial cells （HUVECs） as an alternative cell source. Methods：Biomechanical characteristics of fresh valves and acellularized valves were tested ;HUVECs were cultured in vitro, and frequently seeded onto the acellularized scaffolds, and was their growth observed. Results：The acellularization procedure resulted in complete removal of the cells in valve leaflets while there was no significant difference of biomechanical characteristics between them . The matrix could be successfully seeded with expanded HUVECs in vitro, which formed a continuous monolayer on the surface. Conculsions：Acellularied porcine aortic valves can be applied as a scaffold to create tissue engineering heart valves. Generation of functional tissue engineering heart valves from HUVECs was feasible.