亮菌多糖的纯化工艺及抗肿瘤活性研究

Study on the Technique of Purification and Antitumor Activities of Armillariella Lucidum Polysaccharide

目的:研究亮菌多糖的分离纯化工艺及体内抗肿瘤活性。方法:采用DEAE-纤维素色谱柱对粗品亮菌多糖ALPII进行纯化,以上样回收率为考察指标,通过单因素试验研究不同浓度的NaCl溶液对纯化效果的影响。以抑瘤率为考察指标,采用荷瘤小鼠研究ALP2体内抗肿瘤活性。结果:粗品ALPII经纯化后得到精品多糖ALP1和ALP2;NaCl浓度为0.2mol/L时上样回收率最高,ALP2对实体型肉瘤S180和实体型腹水瘤EAC都有明显的抑制作用。结论:以DEAE-纤维素色谱柱分离纯化粗品亮菌多糖,NaCl的适宜浓度为0.2mol/L;初步药效试验表明ALP2具有抗肿瘤活性。

Objective：To study on purification technology and anti-tumor activity of Armillariella Lucidum Polysaccharide（ALP）.Methods：Crude ALPⅡwere purificated through DEAE-cellulose ion-exchange chromatography.The effect of different concentrations of NaCl was tested by single factorial test.The in vivo antitumor activity was studied on ALP2 Results：Two pure polysaccharide,ALP1 and ALP2 were obtained.The highest recycling rate of Polysaccharide was found by the concentration of 0.2mol/L NaCl.ALP2 can inhibit the growth of S180 and EAC.Conclusions：The optimum concentration of NaCl is 0.2mol/L.The primary effect showed that ALP2 has the anti-tumor activity.