脂肪酶催化动物脂肪水解

Lipase Catalyzed Animal Fats Hydrolysis

研究了脂肪酶ＬｉｐｏｌａｓｅＴＭ催化猪油和牛羊油水解的工艺条件。试验结果表明，适宜的工艺条件是：ｐＨ值８．０，温度５０℃（猪油）或５５℃（牛羊油），底物浓度５１％（Ｗ／Ｖ），搅拌速度４５０ｒ／ｍｉｎ～５００ｒ／ｍｉｎ，酶用量１５０Ｕ／ｇ油脂。在上述条件下水解２４ｈ，水解率＞９５％。

The technology conditions of lipase catalyzed lard and beef-mutton fat hydrolysis has been studied.It is shown that the optimal conditions are pH 8 0,temperature 50 ℃(lard)or 55 ℃(beef-mutton fat),the substrate concentration 52%(W/V),the rate of stirring 450r/min～500r/min and the quantity of lipase 150 U/g (fat).On the above conditions,the hydrolysis ratio of fat is over 95% when lipase catalyzed fat hydrolysis is carried on 24 hours.