KCNA7基因T418M多态性与跑节省化的关联性研究

Study on Association between KCNA7 Gene T418M Polymorphism and Running Economy

目的:通过研究KCNA7基因T418M多态性与耐力训练效果之间的关联性,寻找与耐力训练效果相关的分子遗传学标记;方法:102名无训练史的健康男子以95%～105%个体无氧阈强度进行5000m跑训练,每周3次,共18周的有氧耐力训练,测试训练前后的跑节省化;用PCR-RFLP、基因测序方法研究该基因多态性的分布特征。x^2检验人群是否符合Hardy-Weinberg平衡定律。训练前组间差异及训练变化率用ANOVA分析,两两比较采用Post-Hoc检验。结果:发现该多态的CT型受试者跑节省化提高的训练敏感性较CC型和TT型高。结论:KCNA7基因T418M多态可以用作有氧耐力训练敏感性的分子生物学标记。

This paper studied the associations between KCNA7 gene T418M polymorphism and the effects of endurance training. 102 healthy male subjects with no training history undertook a distance running program consisted of 5000m running with the intensity of 95 % - 105 % anaerobic threshold, 3 times/week for 18 weeks. Running economy （RE） was measured before and after the protocol, and used the PCR-RFLP to explore the association between KCNA7 gene T418M polymorphism and the effects of endurance training. x^2 test was used to determine whether the genotype distribution differed from that expected under the Hardy-Weinberg equilibrium. ANOVA （LSD） tested differences in variables before the training between the three genotypes. Post-Hoc was used to analyze variables differences. The result showed that the running economy of subjects with CT is higher than that of TT and CC. It concludes that KCNA7 gene T418M polymorphism can be used as the molecular biology marker of aerobic endurance training.