摘要
人工采取8只优质犬精液,以果糖-Tris-柠檬酸所组成的稀释液为基础,分别于试验前0.5 h和24 h将OEP,SDS和卵黄混合于基础稀释液中,并于5℃冰箱、22℃两个温度条件下保存,用时离心选取上清液作为稀释剂并制成细管冻精.利用流式细胞术和高倍显微镜对犬冻融精子活力、质膜完整率和顶体完整率等特性进行测定,目的在于比较不同温度条件下OEP,SDS和卵黄相互作用时间的长短对犬冻融精子质量的影响,以探讨OEP,SDS对冻融精子的保护作用机理.添加稀释液前24 h混合OEP和卵黄有利于维持冻融过程中质膜完整率(61.5%)和顶体完整率(66.8%),显著提高冻融精子的总活性比率(20.2%),对犬冻融精子的保护效果明显好于相互作用0.5 h实验组的保护效果(P<0.05);OEP,SDS与卵黄相互作用时的温度对犬冻融精子质量无显著影响(P>0.05),OEP对犬冻融精子的保护效果明显优于SDS.结果提示增加OEP,SDS与卵黄相互作用的时间可以提高犬冻融精子的质量.
The basic extender of Tris-Citric add dilution was added with different concentrations of OEP,SD8 and eggyolk respectively in 0.5 and 24 h before experiment, these two groups were preserved in 5 and 22 ℃ respectively. Semen of eight high quality dogs was diluted with supernatant fluid into 8 groups and preserved in straw. Sperm motility, plasma membrane integrity and acrosomal integrity of frozen-thawed spermatozoa were evaluated using flow eytometry and high power microscope to compare the effects of the interactional time of OEP and SI38 with egg-yolk in different temperature on the quality of dog spermatozoa after freezing-thawing. Results were as following: mixing OEP and egg-yolk in 24 h before added with extender was useful to plasma membrane integrity (61.5 % ) and acrosomal integrity (66.8 % ) of frozenthawed spermatoeoa, the total motility raised prominent (20.2%) and the effect of protection to frozen-thawed sprermatozoa was better than the experimental groups of interactional time was 0.5 h ( P〈 0.05). The temperature of adding OEP and SI38 to egg-yolk had no prominent effect on the quality of frozen-thawed sprermatozoa (P〈0.05). Comparing with SI38, the protecting effect of OEP on frozen-thawed sprermatozoa was excelled obviously. The result showed that increasing the treating time of OEP and SI38 interacting with egg-yolk could increase the quality of frozen-thawed sprermatozxoa.
出处
《复旦学报(自然科学版)》
CAS
CSCD
北大核心
2007年第6期890-895,共6页
Journal of Fudan University:Natural Science
基金
上海交通大学青年基金资助项目(A2122B)
上海交通大学PRP资助项目(T15210006)
关键词
犬
冻融精子
OEP
SDS
卵黄
hoar
spermatozoa
Orvus ES paste
SI38
egg-yolk