粗枝软骨藻Chondria crassicaulis组织培养初探

Primary study on tissue culture of Chondria crassicaulis

在固体培养基上研究了粗枝软骨藻(Chondria crassicaulis)再生的极性,并利用三种不同的培养液和不同的温度对粗枝软骨藻进行切段培养,探索了部分外界因素对粗枝软骨藻生长的影响。发现软骨藻在人工培养条件下的再生出芽过程并无明显极性。在10℃、15℃和22℃三个温度梯度和PES、改良的ASP1和改良的ES三种培养液中,15℃改良的ES培养液中较有利于再生芽的出现。同时我们还观察了粗枝软骨藻四分孢子的发育过程,发现软骨藻具有十字型和四面锥型两种不同的四分孢子囊,软骨藻在四分孢子萌发过程中可形成四种不同的孢子苗。

Regeneration polarity of Chondria crassicaulis was studied in solid medium. We used three different liquid medium and three different temperatures to explore the effects of some external factors to the cultivation of chopped tissues of Chondria crassicaulis. We found that under artificial culture environment there was no obvious polarity in the regeneration of fragments. When cultured in PES, modified ASPs, modified ES culture media and under 10℃, 15℃, 22℃, it was found that the formations of buds were abundant and best in the segments under 15℃ in modified ES liquid media. We also found that there were two kinds of sporangia, cruciform and four conical, in the cultivation, and tetraspore of Chondria cras＇sicaulis could develop into four kinds of seedlings.