多巴胺D2受体和腺苷A2A受体在人视网膜色素上皮细胞表达的研究

Expression of dopamine receptor D2 and adenosine receptor A2A in human retinal pigment epithelium

目的探讨正常人眼视网膜色素上皮（RPE）细胞多巴胺D2受体和腺苷A2A受体表达情况。方法采用已建立的RPE细胞的分离和培养方法建立细胞株。取传3—4代生长良好的细胞，应用逆转录聚合酶链式反应（RT—PCR）、细胞免疫组化方法检测培养的RPE细胞中多巴胺D2受体和腺苷A2A受体的表达情况。结果培养的RPE细胞呈多边形，平均分裂时间为2—3d，细胞内含有棕黄色色素颗粒，随着细胞分裂，细胞内的色素颗粒稀释和变少。RT—PCR和细胞免疫组化检测，显示体外培养的人RPE细胞两种受体均有表达。结论人RPE细胞有多巴胺D2受体和腺苷A2A受体的分布。

Objective The aim of this study was to identify the presence of dopamine receptor D2 and adenosine receptor A2A in human retinal pigment epithelium （RPE） in order to determine whether the RPE is a potential site of action for these two receptors. Methods RPE Cells were isolated and cultured. Reverse transcription-polymerase chain reaction （RT-PCR） and immunocytochemistry were performed to detect the expression of D2 and A2A receptors in the RPE. Results RT-PCR analysis showed that mRNA of D2 and A2A receptors were expressed in the RPE. D2 and A2A receptors were distributed in the RPE by immunocytochemistry. Conclusions This study demonstrated the presence of D2 and A2A receptors in human RPE at both mRNA and protein levels. D2 and A2A receptors may play an important role in the maintenance of the function of human retinal pigment epithelium. Furthermore, their cooperation appeared to be important in the development of axial myopia and retinal and choroidal neovascularization.