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抗hnRNP A2/B1多克隆抗体制备及其在非小细胞肺癌中的应用 被引量：3

The preparation of anti-hnRNP A2/B1 polyclonal antibody and its application in non-small cell lung cancer
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摘要 目的:探讨自主制备抗hnRNP A2/B1多克隆抗体,对非小细胞肺癌(non-small cell lung cancer,NSCLC)的诊断、与病理特征的关系及其在预后中的价值。方法:IPTG诱导表达hnRNP A2/B1蛋白,制备兔抗人hnRNP A2/B1多克隆抗体,在45例NSCLC和16例肺炎性假瘤术后蜡块组织中,采用免疫组化的方法进行检测,并且与国外商品化的抗hnRNP A2/B1单克隆抗体进行比较,以观察自主制备的多抗在临床中的应用价值。结果:成功制备兔抗人hnRNP A2/B1多克隆抗体;应用该多抗在NSCLC组中免疫组化的表达阳性率为62.2%显著高于癌旁组织的40.0%(P=0.035)及炎性假瘤组的31.3%(P=0.033)。与临床病理特征的关系研究中发现,该蛋白高表达与年龄、吸烟呈正相关,与分化程度呈负相关;在预后中2组生存时间有差别,多抗阳性组的生存时间比阴性组的生存时间明显要短(P=0.048)。结论:获得抗hnRNP A2/B1的多克隆抗体,应用该多抗对NSCLC的诊断,病情预后具有一定的临床价值。 Objective： To prepare anti-hnRNP A2/B1 polyclonal antibody and determine its clinical significance in diagnosis and prediction of prognosis of non-small cell lung cancer and analyze the relationship between the expression of hnRNP A2/B1 and pathological features. Methods： HnRNP A2/B1 expression was induced by IPTG. The rabbit-anti-human hnRNP A2/B1 polyclonal anti-bodies were prepared and purified. The specimens were isolated from 45 cases of non small cell lung cancer tissues and paracancerous tissues and 16 cases of lung inflammatory pseudotumor tissues and embedded in paraffin, hnRNP A2/B1 expression was detected by immunohistochemical method using rabbit anti-human hnRNP A2/B1 polyclonal antibodies. The results were compared with imported anti-hnRNPA2/B1 monoclonal antibody to observe the clinical value of self-made polyclonal antibodies. Results： The rabbit anti-human hnRNPA2/B1 polyclonal antibody is successfully generated. The positive rate of hnRNP A2/B1 expression detected by anti-hnRNP A2/ B1 polyclonal antibody was significantly higher in lung cancer tissues than that in paracancerous tissues （62.2% vs 40.0% , P = 0.035 ） and lung inflammatory pseudotumor tissues （62.2% vs 31.3%, P=0.033 ）. The overexpression of hnRNP A2 / B1 was positively related with age and smoking and negatively related with differentiation degree. But it was not related to tumor histological classification, gender, lymph node metastasis, TNM stage, and distant metastasis. （4） The difference in survival time was significant between the two groups, hnRNP A2/B1-positive patients had shorter survival time than hnRNP A2/B1-negative patients （P = 0.048 ）. Conclusions： The rabbit anti-human hnRNP A2/B1 polyclonal antibody is successfully generated. Overexpression of hnRNP A2/B1 in lung cancer tissues has clinical values to a certain degree for diagnosis and prediction of the prognosis of lung cancer.
作者 李业山 雷祖宝 徐美清 李润生 牛俊杨 王晓秋 陈柯 李先武 操乐杰
机构地区 安徽医科大学附属省立医院呼吸科 安徽省合肥市第二人民医院呼吸科 安徽医科大学附属省立医院胸外科 江苏省无锡市第四人民医院呼吸科 安徽医科大学附属省立医院病理科 美国华盛顿大学医学院生物工程系
出处 《肿瘤》 CAS CSCD 北大核心 2007年第12期985-989,共5页 Tumor
基金 安徽省科技厅自然科研基金(编号:050430703) 安徽省科技厅重点科研基金(编号:01023024) 安徽省教育厅自然科学基金(编号:2004kj238zc)
关键词 癌 非小细胞肺 抗体 免疫学技术 Carcinoma, non-small cell lung Antibodies Immunologic techniques
分类号 R734 [医药卫生—肿瘤] R73-3 [医药卫生—肿瘤]
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参考文献7

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共引文献4

同被引文献20

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二级引证文献7

肿瘤
2007年 第12期

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