低浓度神经生长因子存在下GPI-1046刺激鸡胚神经节突起生长(英文)

GPI-1046 stimulates chicken dorsal root ganglion neurite outgrowth in the presence of nerve growth factor at low concentration in vitro

对GPI-1046是否具有神经营养作用目前有两种不同的认识。Steiner等认为GPI-1046能促进体外培养的感觉神经节神经元突起生长。但Harper等却没能证明GPI-1046有这样的作用。由于GPI-1046在临床上具有重要应用价值和前景,我们重新评价了GPI-1046对体外培养鸡胚神经节的神经营养作用,发现在低浓度神经生长因子(nerve growth factor,NGF)存在下,GPI-1046能明显促进鸡背根神经节神经突起的生长。

The purpose of this investigation was to re-evaluate the neurotrophic effect of GPI-1046 on neurite outgrowth in vitro. GPI- 1046 was synthesized and identified with mass spectrometry, nuclear magnetic resonance and elemental analysis. Chicken dorsal root ganglions （DRGs） were removed and divided into three groups： （1） The DRGs were cultured in DMEM containing different concentrations of GPI-1046; （2） The DRGs were cultured in DMEM containing nerve growth factor （NGF） alone at 0.8 and 8 ng/mL, respectively; （3） The DRGs were cultured in DMEM containing both different concentrations of GPI-1046 and NGF at 0.8 ng/mL. The results showed that GPI-1046 alone could not stimulate chicken DRG neurite outgrowth; however, GPI-1046 stimulated DRG neurite outgrowth only in the presence of NGF at low concentration in the culture medium.