丘脑底核高频电刺激对猴帕金森病模型氨基酸类神经递质的影响

Changes of amino acids neurotransmitters in striatum of hemiparkinsonian rhesus monkev after high frequency stimulation of subthalamic nucleus

目的探讨长期丘脑底核高频电刺激对猴帕金森病模型纹状体细胞外液中氨基酸类神经递质含量的影响。方法成功制作猴右侧帕金森病丘脑底核高频电刺激模型2只,分别在打开脉冲发生器前和开机后的不同时间点应用微透析的方法对纹状体细胞外液进行取样(开机后1周、1、8、12个月)。应用高效液相色谱荧光法检测开机前后的尾状核和壳核细胞外液的谷氨酸、γ-氨基丁酸、牛磺酸的含量变化。结果在长期高频电刺激下,电极侧壳核和尾状核的谷氨酸含量在开机后的各时间点较开机前明显增加。电极侧尾状核及壳核的γ-氨基丁酸含量在开机后1周、1个月时均较开机前明显增加(P<0.05),但在开机后8、12个月时γ-氨基丁酸含量较开机前、开机1周、1个月时又明显下降(P<0.05)。电极侧壳核和尾状核的牛磺酸含量在开机后较开机前明显增加(P<0.05)。结论丘脑底核长期高频刺激可以增加纹状体区细胞外液中谷氨酸和牛磺酸的含量,明显降低γ-氨基丁酸的含量,改善偏侧猴帕金森病模型的症状。

Objective To investigate the influence of high frequency stimulation of the subthalamic nucleus on the levels of amino acids neurotransmitters in striatum of hemiparkinsonian monkeys. Methods Two rhesus monkeys were successfully prepared for the subsequent microdialysis sessions. Collecting the dialyate before turning on the pulse generator, and collecting at 1 week, 1, 8 and 12 months after high frequency stimulation of the subthalamic nucleus. The level of Glu, GABA and Tan were determined by high performance liquid chromatography and fluorometric detection （ HPLC-FD ）. Results After high frequency stimulation （HFS）, PD symptoms of monkeys significantly improved. The levels of Glu in putamen and caudate nucleus of electrodes side at 1 week,1, 8 and 12 months were increased significantly. The levels of GABA in putamen and caudate nucleus of electrodes side at 1 week, 1 month increased significantly compared with before turning on the pulse generator while decreased at 8, 12 months. The level of Tan in putamen and caudate nucleus increased significantly. Conclusion Long term STN HFS can increase the level of glutamate and taurine, while decrease the level of GABA in putamen and caudate nucleus of electrodes side. It improves symptoms of hemiparkinsonian rhesus monkey significantly.