摘要
采用RT-PCR技术从野生种马铃薯中克隆到一个光敏色素基因PHYB,其cDNA全长为3470bp。含有一个3393 bp的完整开放阅读框,编码一条长1130个氨基酸的蛋白,分子量为125kDa,等电点为5.6。该基因编码的蛋白序列与栽培种马铃薯、番茄和烟草同源基因编码的氨基酸序列一致性分别为98%、95%、92%,命名为SpPHYB.半定量PCR分析表明,根、茎、叶和芽中SpPHYB表达水平较高且相似,但在花和块茎成熟器官中表达量稍低.
The full-length cDNA of SpPHYB gone was cloned from wild potato （Solanum pinnatisectum） by RT-PCR. The cDNA was 3 470 bp long with an open reading frame （ORF） of 3 393 bp and encoded 1 130 amino acid residues with a predicted molecular weight of 125 kDa and an isoelectric point of 5.6. The protein sequence comparison showed that the predicted protein had higher identifies to those from Solanum tuberosum, Lycopersicon esculentum and Nicotiana tabacum （98%, 95% and 92%）. Semi-quantitive RT-PCR indicated that the expression level of SpPHYB was higher and similar in roots, stem, leaves and sprouts, and lower in flowers and tubers.
出处
《植物生理学通讯》
CSCD
北大核心
2007年第6期1020-1024,共5页
Plant Physiology Communications
基金
国家转基因植物研究与产业化开发专项(JY03-A-11)
