摘要
HbMyb1是橡胶树死皮病相关基因,为了进一步研究HbMyb1的功能,采用酵母双杂交方法,从胶乳cDNA文库中筛选出10个与HbMyb1发生相互作用的蛋白,包括已知的橡胶延伸因子和橡胶小粒子蛋白等,其中4个分别与3-羟酰基-ACP-脱氢酶、NAD-依赖的山梨糖醇脱氢酶、氧化还原酶、假定的富含亮氨酸重复蛋白具有同源性的蛋白,其余4个为未知蛋白。
HbMyb1 was related to TPD （tapping panel dryness） of Hevea brasiliensis. To study the function of HbMyb1 protein, we used yeast two-hybrid assay to screen a latex cDNA library with full-length of HbMyb1. Ten proteins interacted with HbMyb1 protein were obtained. Among them, 6 proteins with known functions were obtained, including the rubber elongation factor, small rubber particle protein, 3-hydroxyacyl-acyl-carrier protein dehydratase, NAD-dependent sorbitol dehydrogenase enzyme, oxidoreductase and putative leucine-rich repeat protein. 4 proteins with unknown function were found. These work could help for the further study on the function of HbMyb1 and the clarification of mechanism of the TPD.
出处
《植物生理学通讯》
CAS
CSCD
北大核心
2007年第6期1031-1034,共4页
Plant Physiology Communications
基金
中国热带农业科学院科技基金(Rky050103)
关键词
巴西橡胶树
死皮病
HbMyb1
酵母双杂交
Hevea brasiliensis
tapping panel dryness （TPD）
HbMyb1
yeast two-hybrid system
