摘要
水杨酸(SA)可以浓度依赖的方式诱导蚕豆叶片的气孔关闭,1～1000μmol·L^(-1)SA所诱导的气孔关闭可以再开放,而10^(-2)mol·L^(-1)的SA导致的气孔关闭则否。质膜NADPH氧化酶抑制剂二亚苯基碘(DPI)可削弱SA作用的45%～60%。表明SA诱导的气孔关闭可能与H_2O_2的产生有关。以H_2O_2荧光探针H_2DCFDA结合显微注射技术直接检测保卫细胞内产生H_2O_2的结果显示,100μmol·L^(-1)SA可引起保卫细胞内荧光素(DCF)荧光快速增强。在DPI存在的情况下,经SA处理的保卫细胞,仅在其叶绿体部位产生H_2O_2,而质膜附近的DCF荧光增强则受到抑制。表明叶绿体可能是保卫细胞内产生H_2O_2的主要部位,质膜NADPH氧化酶也可能参与SA诱导H_2O_2的产生。
Salicylic acid （SA） can induce stomatal closure with a concentration-dependent manner, the closed stomata can open again when the concentration of SA is between 1-1 000 μmol·L^-1, but when the concentration of SA is 0.01mol·L^-1, the change of closed stomata cannot happen. DPI 10 μmol·L^-1 weakened the effect of SA on stomatal apertures by 45%-60%, which indicated that the generation of H2O2 might be involved in the stomatal closure induced by SA. We directly examined the production of H2O2 by microinjection based on H2DCFDA. SA 100 μmol·L^-1by applied externally or microinjection could induce rapid increasing in fluorescence of DCF in guard cells. In the presence of DPI, the guard cells treated with SA 100μmol·L^-1 showed increase in DCF fluorescence only in the regions of chloroplasts, but the production of H2O2 was inhibited in the regions of plasma membrane. These results suggest that the chloroplasts in guard cells might be the main source of H2O2 production, meanwhile, plasma membrane NADPH oxidase may also contribute to the H2O2 generation in the guard cells, when treated with SA.
出处
《植物生理学通讯》
CSCD
北大核心
2007年第6期1040-1044,共5页
Plant Physiology Communications
基金
国家自然科学基金(39870372)
