生长调节物质、碳源和光周期对山薯试管薯形成和生长发育的影响

Effects of Growth Regulators,Carbon Sources and Photoperiod on in vitro Formation and Growth and Development of Microtubers of Dioscorea fordii Prain et Burk.

研究了IBA、NAA、PP_(333)和KT这4种植物生长调节剂组合处理以及碳源与光周期处理对山薯试管薯形成和生长发育影响。结果表明:(1)MS+2.0mg·L^(-1)IBA+1.0mg·L^(-1)NAA+0.5mg·L^(-1)PP_(333)+1.0mg·L^(-1)KT有利于山薯试管薯的形成和生长发育(成薯指数18.2189);(2)蔗糖作为碳源对山薯试管薯数量的诱导效果优于白糖(每瓶试管薯增加8.20个):75g·L^(-1)的糖有利于山薯试管薯的形成和生长发育(成薯指数225.5590);(3)光照时间长短影响试管薯数量,但对成薯指数的影响不显著。

Taking tissue-cultured plantlets of Dioscorea fordii as material, effects of plant growth regulators （IBA, NAA, PP333 and KT）, carbon sources and photoperiod on formation and growth and development of microtuber of Dioscoreafordii were studied. Results showed that： （1） MS＋2.0 mg·L^-1 IBA＋1.0 mg·L^-1 NAA＋0.5 mg·L^-1 PP333＋1.0 mg·L^-1 KT was helpful to microtuber formation and development of Dioscoreafordii, with the average tuber-producing-index of 18.2189. （2） Sucrose was much better than white sugar as carbon sources for inducement of tuber, the average number per bottle of tuber increased 8.20; sugar of 75 g·L^-1 was helpful to microtuber formation and development of Dioscorea fordii, with the average tuber-producing-index of 225.5590. （3） Length of illumination time influenced the number of tuber, but had no significant influence on tuberproducing-index.