摘要
目的建立并评价微孔板AlamarBlue和MTT分析法用于抗结核分枝杆菌药物最低抑菌浓度（MlC）的测定。方法应用AlamarB1ue和MTr显色法将12种抗结核药物对结核分枝杆菌H37Rv‰的最低抑菌浓度分别进行测定，并与传统的试管二倍稀释法进行比较。结果AlamarBlue和MTr试验分别在6天和8天报告结果，Alamarblue法与试管法相比较，完全符合率是91．7％（11/12）：MTT法与试管法相比较完全符合率是75％（9／12），具有较好的一致性。结论AlamarBlue和MTT方法测定药物对结核分枝杆菌的MIC具有快速、操作简便等特点，适用于新抗结核化合物的大量快速筛选。
Objective To set up and assess use of MTr and Alamar blue assay methods for determining the minimum inhibitory concentration （M1C） of anti-tuberculoisis drug for Mycobocterium tuberculosis. Method The M1C of 12 anti-tuberculosis agents to H37Rv were determined respectively by MTT and Alamar Blue assay methods and compared with the conventional two folds dilutions method. Results For MTr and Alamar blue assays , the time for obtaining results was 7 days and 10 days respectively. The MICs determined by either MTT or Alamar blue assay were highly correlated with those by the two folds dilutions. The determinate corresponding rate of Alama Blue assay was 97.7%（11/12）,MTr assay was 75%（9/12）. Conclusion Alamar blue and MTr for anti-tuberculosis drugs assays are sensitive, rapid, inexpensive, and offers the potential for performing rapid high - throughout scrEening of compounds against Mycobacterium tuberculosis.
出处
《结核病与胸部肿瘤》
2007年第4期251-254,共4页
Tuberculosis and Thoracic Tumor
