PTEN基因在非小细胞肺癌中表达的临床研究

Expression of PTEN Gene in Non-Small Cell Lung Cancer

目的研究PTEN基因在人非小细胞肺癌中表达情况，探讨PTEN基因在人肺癌发生、发展过程中的可能作用。方法采用免疫组化方法检测143例非小细胞肺癌、20例正常肺组织及良性病变中PTEN蛋白表达情况。结果在正常肺组织及肺良性病变组织中无失表达：肺癌组织中失表达率57．34％（82／143），两者比较有显著性差异（P〈0．001）；肺癌组织中PTEN基因表达水平与肿瘤细胞分化程度、TNM分期、淋巴结转移程度存在相关性（P〈0．001）；PTEN蛋白失表达组．肺癌患者的术后五年生存率显著低于表达组（P〈0．001）；吸烟组患者PTEN基因失表达率显著高于无吸烟组（P〈0．001）。结论PTEN蛋白失表达与肺癌的发生、发展及预后有关；多因素分析PTEN基因失表达是影响非小细胞肺癌预后的独立因素。

Objective To investigate the expression of PTEN gene in non-small cell lung cancer （NSCLC）, and to analyze the role of PTEN gene in lung tumorigenesis. Methods Immunohistochemical stain was used to determine the expression of PTEN in 20 eases of normal lung tissues and benign pulmonary lesion tissues and 143 cases of non-small cell lung cancer. Results The negative rate of PTEN expression in normal lung tissues and benign pulmonary lesion tissues was 100.00% which was significantly higher than that in tissues of non-small cell lung cancer 57.34%（P〈0.001）.The expression of PTEN gene was closely associated with cell differentiation, TNM stages and lymph node metastasis in lung cancer patients （P〈0.001）. A marked reduction of PTEN protein expression was observed more frequently in patients with smoking history than in those not smoking （P= 0.003）. Furthermore, FTEN protein expressing level in non-small cell lung cancer was associated with a poorer survival of patients （P〈0.001）. Conclusion These results suggest that loss of HEN protein expression can be concerned in carcinogenesis of NSCLC and be an independent poor prognostic factor.