Nr-CWS对TC-1细胞致瘤作用的影响及抗女性下生殖道人乳头状瘤病毒感染的研究

Effect of Nocardia rubra cell wall skeleton (Nr-CWS) on oncogenicity of TC-1 cells and anti-human papillomavirus effect of Nr-CWS in lower genital tract of women

目的探讨Nr-CWS对含HPV16型病毒基因的TC-1细胞荷瘤鼠肿瘤生长的影响及对女性下生殖道HPV感染的清除效果。方法SCID小鼠分别注射TC-1细胞形成肿瘤模型后随机分成3组,分别注射生理盐水、60μg/ml和120μg/mlNr-CWS,观察肿瘤生长的情况。临床选取45例单纯HPV感染者使用Nr-CWS1个月、3个月后再次检测HPV,分析Nr-CWS对生殖道HPV感染的清除效果。结果动物实验表明两组用药组的肿瘤体重明显低于对照组,抑瘤率分别为59.1%和84.2%;三组瘤重用秩和检验分析P<0.05,差异有统计学意义;临床实验结果显示用药后3个月HPV检测转阴23例(51.1%),型别减少9例(20%),无效13例(28.9%);治愈率与自然转阴率31%相比较,χ2检验P=0.005,差异有统计学意义。结论Nr-CWS对HPV16型病毒基因的TC-1细胞荷瘤鼠肿瘤有明显的抑制作用;且对临床女性下生殖道HPV感染有一定的清除作用。

Objective To detect the effect of Nocardia rubra cell wall skeleton （Nr-CWS） on tumorigenicity induced by TC-1 cells and to Clinically study anti-human papillomavirus effect of Nr-CWS in lower genital tract of women. Methods Tumor model was estabhshed by injecting TC-1 cells subcutaneously in SCID mice, then divided them into 3 groups randomly and injected with isovolumetric physiological saline, 60 μg/ml NrCWS and 120 μg/ml Nr-CWS respectively, the growth of tumors was measured one week later. Nr-CWS was applied on 45 HPV positive women whose TCT test was normal and without cervical erosion 2-3 days after menstruation. HPV was detected again 3 months later to explore the effect of Nr-CWS on HPV infection in female lower genital tract. Results The animal experiment showed the weight of transplanted tumors in treated group was less than that of control group （ X^2 = 12.5, P = 0.002）. The tumor inhibition rate was 59.1% and 84.2% in the groups treated with Nr-CWS 60 and 120 gg/ml Nr-CWS; the results of HPV detection in 23 out of the 45 cases （51.1% ） became negative after the 3-month treatment; the viral load was reduced in 9, and there was no change in viral load in 13 cases. Significant difference was found between the rates of undetectable viral load and the natural viral disappearance rate （ P 〈 0.05 ）. Conclusions Nr-CWS has an inhibitory effect to TC-1 cell tumorigenesis and clinical application of Nr-CWS may eliminate the HPV infection in lower genital tract of a considerable proportion of women with HPV infection.