慢性丙型肝炎患者HCV血清学分型研究

Concordance between hepatitis C virus serotype and genotype in chronic hepatitis C patients in China

目的探讨HCV基因分型与血清学分型的关系。方法对来自14家医院的104例已知HCV基因型的慢性丙型肝炎患者血清,经ELISA方法,用Murex HCV Serotyping1-6 Assay血清分型试剂进行HCV的血清学分型。结果104例血清中的86(82.69%)例可分出血清型,检出血清型病毒株91株,病毒株检出率为78.4%。血清型与基因型的总符合率为62.1%,血清型1型、2型和3型的符合率分别为69.4%、51.2%和70.0%,以2b基因型的符合率和漏检率最低(54.5%)。结论HCV血清型特异性受病毒基因型的影响,与基因型存在一定的差异。

Objective To investigate the relationship of hepatitis C virus （HCV） semtype with genotype. Methods The semtypes of HCV in the serum of 104 patients with chronic hepatitis C from 14 cities in China for which HCV genotypes were available, were determined by ELISA using Murex HCV Semtyping 1-6 Assay. Results The semtypes of 86 （82.69%） of the 104 serum specimens were determined, and HCV semtypes were determined for 91 strains. Overall the concordance between hepatitis C virus semtype and genotype was 62.1%, and the concordance of semtype, with genotypes 1, 2 and 3 were 69.4%, 51.2% and 70.0%, respectively. The false-negative rate and concordance of genotype 2b was lower （54.5%）. Conclusion The specificity of HCV semtyping was affected by HCV strains＇ genotype and sometimes HCV serotype was not in concordance with genotype.