不同倍性何首乌中二苯乙烯苷和蒽醌类的含量比较

Comparison of Content of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside and Anthraquinon in Different Ploidy Radix Polygoni Multiflori

目的:考察二倍体与三倍体何首乌中二苯乙烯苷、蒽醌类成分的含量差异。方法:采用Inertsil ODS-3C18柱,分别用乙腈-水(25∶75)、甲醇-0.1%磷酸(85∶15)作为流动相,在320 nm和254 nm处检测2,3,5,4′-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、蒽醌类成分的含量。结果:广东德庆二倍体何首乌中二苯乙烯苷的含量最高,广西靖西三倍体何首乌中结合型蒽醌的比例达到了85%。结论:不同倍性何首乌中化学成分存在差异。

Objective： To investigate the content of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucoside and anthraquinon in diploid and triploid Radix Polygoni Muhiflori. Methods： 4 batches of Radix Polygoni Multiflori were collected from different districts. The content of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucoside and anthraquinon in these samples were determined at 320nm and 254nm wave length by HPLC with Inertsil ODS-3 C18 pillar and acetomitrile： aqua （25：75） , methanol： 0. 1% phosphoric （85：15 ） respectively as the mobile phase. Results： The maximum content of 2,3,5,4＇-tetrahydroxystilbene-2-O-β-D-glucoside was diploid Radix Polygoni Multiflori from Deqing Guangdong, The maximum ratio of total anthraquinon was triploid Radix Polygoni Multiflori from Jinxi Guangxi reached 85%. Conclusion： The content of anthraquinon varies greatly in the samples from the different producing areas.