摘要
采用顶空固相微萃取-气相色谱-质谱联用法(HS-SPME-GC-MS)分离,鉴定了松花粉中的挥发性成分。实验中筛选了固相微萃取纤维,优化了SPME的操作条件。样品在70℃下,用100μm聚丙烯酸酯(PA)固相微萃取纤维对松花粉样品顶空吸附30 min,于250℃脱附10 min然后采用GC-MS对解析物进行分离鉴定。采用HS-SPME-GC-MS测得85个峰,鉴定出70种组分,主要为脂肪类化合物。结果表明:HS-SPME可用于松花粉中挥发性成分的快速分析。
Headspace solid-phase microextraction （HS-SPME） technique was employed to extract the volatile compounds from Pollen Pini. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry （GC-MS）. The volatiles were collected using several SPME parameters（ including extraction time and temperature）. The best response was obtained using a PA fiber by when the extraction temperature was 70℃, extraction time was 30 minutes and desorption time was 10 time. Analysis was performed by GC-MS. Seventy components were identified by HS-SPME. The main components were fatty compounds. The results showed that the HS-SPME technique could be used to extract the volatile components from HS-SPME-GC-MS.
出处
《中药材》
CAS
CSCD
北大核心
2007年第12期1521-1525,共5页
Journal of Chinese Medicinal Materials
关键词
顶空固相微萃取
顶空萃取
气相色谱-质谱联用
挥发性成分
松花粉
Headspace solid-phase microextraction （HS-SPME）
Headspace extraction
Gas chromatography-mass spectromentry
Volatile components
Pollen Pini
