葡萄籽原花青素对原代培养海马细胞氧化应激的影响

Effects of Grape Seed Proanthocyanidins on Oxidative Stress in Primary Rat Hippocampal Neurons

目的:研究葡萄籽原花青素(GSP)对过氧化氢(H2O2)诱导大鼠原代培养海马细胞氧化应激损伤的影响。方法:采用原代培养的方法,建立H2O2致海马细胞氧化损伤模型,观察细胞形态学变化;MTT法测定细胞活力;化学比色法测定细胞内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性,以及活性氧(ROS)、丙二醛(MDA)含量。结果:1 mmol/L的H2O2诱导海马细胞24 h,细胞活力下降(均P<0.01),细胞内ROS和MDA含量增加,SOD、CAT及GSH-Px活性下降。而GSP可明显减少细胞损伤,使细胞内SOD、CAT及GSH-Px活性增加,并减少细胞内ROS和MDA含量。结论:GSP对H2O2诱导的海马神经细胞氧化应激损伤具有保护作用,其作用机制可能与提高海马细胞的抗氧化能力有关。

Objective： To investigate the effects of grape seed proanthocyanidins（GSP） on the oxidative stress induced by hydro- gen peroxide （H2O2 ） in primary rat hippocampal neurons. Methods： Primary cultured hippocampal neurons injury model by H2O2 was established. Morphological changes of neuron cells were visualized. The viability of hippocampal neurons was detected by MTr. The contents of malonidaldelyde（MDA） , reactive oxygen species （ROS） , superoxide dismutase （SOD） , catalase （CAT） and glutathione peroxidase （GSH-Px） in the cells were measured by chemochromatometry respectively. Results： The cell activity decreased remarkably induced by 1 mmol/L H2O2 for 24 hours （P 〈0.01 ） , the levels of MDA and ROS were increased, and SOD, CAT, GSH-Px level were decreased in cells. GSP could reduce MDA and ROS level obviously, and increase SOD, CAT and GSH-Px level in cells. Conclusion： GSP has protective effect on hippocampal neurons injury induced by H2O2. The protective effect may he related to protecting cell membrane, increasing the activity of clearance enzyme of free radical and inhibiting lipid peroxidation.