利用活性部位指纹图谱筛选驱虫斑鸠菊提取工艺

Application of Fingerprint of Active Part on Extraction Techniques for Vernonia anthelmintica

目的:以活性部位指纹图谱为模板,探讨建立驱虫斑鸠菊提取工艺的可行性。方法:采用正交实验设计,以活性部位的HPLC指纹图谱色谱峰的变化为指标,应用多指标实验公式评分法考察提取工艺条件。结果:驱虫斑鸠菊的最佳提取工艺为,加入10倍量水,浸泡1 h,煎煮1 h,共3次。结论:所选工艺条件具有科学、简单、实用的特点。

Objective ： To probe into feasibility of extraction techniques for Vemonia anthelmintica using HPLC fingerprint of active part as model. Methods： The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices. Results ： The optimum extracting procedure was as follows： using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each. Conclusion： It is scientific, simple and applicable.