丹参联合aFGF对新生缺氧缺血性脑损伤大鼠大脑的保护作用

The protective role of salvia miltiorrhiza plus aFGF in the brain injured by the shortage of blood and oxygen in baby rats

目的观察丹参联合酸性成纤维细胞生长因子(aFGF)对新生缺氧缺血性脑损伤(HIBD)大鼠大脑的保护作用,旨在为该病临床药物治疗提供理论依据。方法制作新生HIBD大鼠模型后给予丹参、aFGF和丹参+aFGF,观察血清中超氧化物歧化酶(SOD)、一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)的水平变化;计数病变大脑组织中血管内皮生长因子(VEGF)阳性细胞数、新生血管和脑皮层的凋亡细胞数。结果(1)空白对照组中NO和iNOS含量明显增多,使用丹参后NO和iNOS下降,与空白对照组比较差异有统计学意义(P<0.01)。(2)正常对照组和空白对照组中新生血管数和VEGF阳性细胞数少,使用aFGF后两者明显增多,丹参+aFGF组两者数量增多,与丹参组及aFGF组比较差异有统计学意义(P<0.01)。(3)使用丹参或aFGF后脑皮层的细胞凋亡细胞数明显减少,与空白对照组比较有统计学意义(P<0.01);丹参+aFGF组脑皮层的细胞凋亡细胞数减少更明显。与丹参组、aFGF组比较差异有统计学意义(P<0.01)。结论复方丹参注射液联合aFGF治疗新生HIBD大鼠在保护脑功能、促进血管增生方面有一定的协同作用。

Objective To observe the protective role of salvia miltiorrhiza plus aFGF in the brain injured by the shortage of blood and oxygen in baby rats. Methods Salvia miltiorrhiza, aFGF or salvia miltiorrhiza ＋ aFGF were given to the rats with brain injured by the shortage of blood and oxygen, and then serum levels of SOD, NO and iNOS were measured and numbers of VEGF positive cells in brain vessel endothelium, new formed vessels and apoptosis cells in brain were counted. Results （1）The serum levels of NO and iNOS in the salvia miltiorrhiza treated groups were lower than those in the control group, P〈0.01. （2） The numbers of VEGF positive cells and new formed vessels were higher in thesalvia miltiorrhiza ＋ aFGF group than those in the salvia miltiorrhiza group, aFGF group or the control groups, P 〈0.01. （3） The numbers of the apoptosis cells were reduced by the treatment ofsalvia miltiorrhiza or aFGF. There were less apoptosis cells in thesalvia miltiorrhiza ＋ aFGF group than in the salvia miltiorrhiza group, aFGF group or the control groups, P 〈 0.01. Conclusion Salvia miltiorrhiza plus aFGF may play a role in protecting the brain function of HIBD baby rats and promoting the regeneration of vessel.