高碘对体外培养成纤维细胞增殖活性的影响

Effect of High Iodine Level on Proliferation of Cultured Fibroblast

目的研究不同浓度碘对体外培养的人皮肤成纤维细胞增殖活性的影响。方法将体外培养的成纤维细胞(HSF)分为9组,[1个空白对照组(0μg/L),8个实验组(100、500、1000、3000、5000、7000、9000、11000μg/L),每组6个平行样]。应用含不同浓度碘化钾的DMEM培养基连续培养24h,观察成纤维细胞的密度和形态的变化并采用四唑盐(MTT)比色试验检测细胞增殖活性。结果碘的浓度在5000μg/L以下时,成纤维细胞的增殖活性随碘浓度的升高而增加;5000、7000μg/L组细胞增殖活性最高,两组间增殖活性差异无统计学意义(P>0.05);7000、9000、11000μg/L组细胞增殖活性随碘浓度的升高而降低。结论碘的浓度在5000μg/L以下,具有促进成纤维细胞增殖的作用;高于7000μg/L时,会抑制成纤维细胞的增殖活性。

Objective To study the effects of iodine on the proliferation activity of fibroblasts at different doses. Methods The cultured fibroblasts were treated with different dose iodine（100 μg/L, 500 μg/L 1 000 μg/L 3 000 μg/L 5 000 μg/L, 7 000 μg/L, 9 000 μg/L, 11 000 μg/L）for 24 h and observe the morphology offibroblast. MTF colorimetry was used to detect the fibroblast proliferation activity. Results Iodine could significantly increase the fibroblast proliferation activity at certain range of concentration （〈5 000 μg/L）, but decrease the proliferation activity at certain range of concentration （〉7 000 μg/L）. The proliferation activity was highest from 5 000 μg/L to 7 000 μg/L, the differences were significant compared with the iodine free control. Conclusion Iodine, the exposure level is 〈5 000 μg/L can increase the fibroblast prohfemtion activity, whereas decrease the activity as the exposure level is 〉7 000 μg/L.