化妆品中甲基异噻唑啉酮及其氯代物的高效液相色谱测定法

Determination of Methylisothiazolinone and Chloro-methylisothiazolinone in Cosmetics by High Performance Liquid Chromatography

目的建立化妆品中甲基异噻唑啉酮及其氯代物的高效液相色谱法测定分析方法。方法样品以甲醇为溶剂,经超声提取,以甲醇-水(体积比为25∶75)为流动相进行分离,流速为1.0ml/min,二极管阵列检测器在276nm波长处检测。结果氯化甲基异噻唑啉酮和甲基异噻唑啉酮的线性范围分别为0.05～100μg/ml和0.0125～25μg/ml,方法检出限分别为0.20和0.08μg,实际样品的加标回收率分别为88.3/～92.9/和84.5/～93.9/,RSD分别为2.9/～5.8/和2.5/～9.4/(n=6)。结论该方法简便、快速、准确,可用于化妆品中甲基异噻唑啉酮及其氯代物的分析测定。

Objective To develop a high performance liquid chromatography method for the determination of methylisothiazolinones and chloro-methylisothiazolinoe in cosmetics. Methods The cosmetic samples were extracted with methanol. The analytes were separated with the mobile phase of V（Methanol）：V（Water）= 25：75 at the flow rate of 1.0 ml/min and detected at the wavelength of 276 nm. Results The linear range of the method was 0.05-100 mg/ml for methylisothiazolinone and 0.0125-25 mg/ml for chloro-methylisothiazolinoe. The limit of detection of methylisothiazolinone and chloro-methylisothiazolinoe were 0.08 and 0.2 μg respectively. The recovery rates were 84.5%-93.9% with the RSD of 2.5%-9.4% （n=6） for methylisothiazolinone and 88.3%- 92.9% with the RSD of 2.9%-5.8% （n=6） for chloro-methylisothiazolinoe. Conclusion The method is simple, rapid, accurate and is suitable for the determination of methylisothiazolinone and chloro-methylisothiazolinoe in cosmetics.