肿瘤坏死因子与肾小球系膜细胞的关系

The relationship between tumor necrosis factor and glomerular mesangial cells.

目的研究肾小球系膜细胞的肿瘤坏死因子的自分泌功能，肿瘤坏死因子对肾小球系膜细胞作用的机制。方法利用体外培养的人和大鼠的肾小球系膜细胞，通过逆转录－多聚酶链反应、原位杂交和免疫组化方法，探讨它们之间的关系。结果肾小球系膜细胞既可产生肿瘤坏死因子，又有肿瘤坏死因子受体。结论肾小球系膜细胞是肿瘤坏死因子作用的靶细胞，肿瘤坏死因子通过旁分泌和自分泌两种途径作用于肾小球系膜细胞。

Objective To study the autocrine function of tumor necrosis factor (TNF) in glomerular mesangial cells (MC) and the action of TNF on MC. Methods Cultured rat and human MC were studied by RT PCR, in situ hybridization and immunohistochemical methods. Results The expression of both TNF and TNF receptor mRNA and protein synthesis in MC were manifested. Conclusion (1) MC is the target cell for TNF, MC have specific cell surface receptor to TNF. (2) TNF acts on MC through both autocrine and paracrine mechanism.