肺淋巴上皮瘤样癌与EB病毒的相关性

Association of Epstein Barr virus with lymphoepithelioma like carcinoma of the lung.

目的为阐明肺淋巴上皮瘤样癌（ｌｙｍｐｈｏｅｐｉｔｈｅｌｉｏｍａ－ｌｉｋｅｃａｒｃｉｎｏｍａ，ＬＥＬＣ）与ＥＢ病毒（ＥＢＶ）的关系。方法对３０例肺ＬＥＬＣ和１９例肺非ＬＥＬＣ的石蜡包埋组织，采用原位杂交、免疫组化技术检测ＥＢＶ的存在情况及其基因表达特征。结果肺ＬＥＬＣ中原位杂交ＥＢＶ小ＲＮＡ（ＥＢＥＲｓ）阳性率为９３．３％（２８／３０）；而免疫组化潜在膜蛋白（ｌａｔｅｎｔｍｅｍｂｒａｎｅｐｒｏｔｅｉｎ，ＬＭＰ１）阳性率分别为５３．３％（１６／３０）和２３．３％（７／３０）；１９例病毒衣壳抗原（ｖｉｒｕｓｃａｐｓｉｄａｎｔｉｇｅｎ，ＶＣＡ）阳性率为２３．３％（７／３０）；１９例非ＬＥＬＣ肺癌中原位杂交检测ＥＢＥＲｓ、免疫组化检测ＬＭＰ１均为阴性。结论肺ＬＥＬＣ与ＥＢＶ密切相关，ＥＢＶ可能在肺ＬＥＬＣ发生发展中起重要的作用；ＥＢＶ在肺ＬＥＬＣ中主要以潜伏状态存在，只有少量病毒进入溶解周期。

Objective To study the relationship between pulmonary lymphoepithelioma like carcinoma (LELC) and Epstein Barr virus (EBV). Methods 30 cases of LELC and 19 cases of non LELC pulmonary pulmonary specimens were studied by in situ hybridization and immunohistochemistry techniques to detect EB virus small RNA (EBERs), latent menbrane protein 1 (LMP 1) and virus capsid antigen (VCA). Results The detection rates for EBER, LMP 1 and VCA were 93.3% (28/30), 53.3%(16/30) and 23.3%(7/30) respectively. LMP 1 positive carcinoma cells were not found in the 19 non LELC specimens, which were used as the controls. Conclusion Pulmonary LELC is closely associated with latent EBV infection and may be an important factor involved in the development and progression of LELC. In addition, EBV lytic gene products may sometimes be found in a small portion of LELC cells.