涎腺良恶性淋巴上皮病变中EB病毒DNA　及其产物检测的意义

The Significance of detecting EB virus and its products in benign and malignant lymphoepithelial lesions of the salivary glands.

目的了解良恶性淋巴上皮性病变与埃泼斯坦－巴尔（ＥＢ）病毒的关系。方法采用原位杂交、聚合酶链反应（ＰＣＲ）及免疫组化ＬＳＡＢ法对涎腺１８例恶性淋巴上皮病变（ＭＬＥＬ）、１４例良性淋巴上皮病变（ＢＬＥＬ）进行ＥＢＶ编码的ＲＮＡ（ＥＢＥＲ１）、ＥＢＶＢａｍＨ１Ｗ片段及ＥＢＶ潜在膜蛋白（ＬＭＰ１）和潜在膜抗原（ＥＢＮＡ２）检测。结果（１）本组１８例ＭＬＥＬＥＢＥＲ１和ＥＢＶＢａｍＨ１Ｗ片段均为阳性，１４例ＢＬＥＬ则为阴性。（２）１８例ＭＬＥＬＬＭＰ１检出率７７．８％（１４／１８），ＥＢＮＡ２未检出（０／９）。（３）ＭＬＥＬ间质淋巴细胞多数为Ｔ细胞，ＢＬＥＬ间质多为Ｂ细胞。（４）本组统计２１６例涎腺癌中有３７例ＭＬＥＬ，发生率为１７．１％。结论本结果表明ＥＢＶ与ＭＬＥＬ存在密切关系，很可能在其发病中起着重要作用。文中还对ＢＬＥＬ与ＥＢＶ的关系、ＢＬＥＬ与ＭＬＥＬ的关系进行了讨论。

Objective To study the relationship between EB virus and benign malignant lymphoepithelial Lesions (ELEL) and malignant lymphoepithelial lesions (MLEL). Methods In situ hybridization, polymeras Chain reaction (PCR) and immunohistochemical methods were used to detect EBV DNA, EBER1 and latent membrane protein 1 (LMP1) on paraffin embedded tissues of 18 MLEL cases and 14 BLEL cases. Results (1) Positive rates for Bam H1 W fragmet by PCR and EBER1 by in situ hybridization in 18 cases of MLEL were both 100%. Positive rates of EBV DNA by PCR and EBER1 by in situ hybridization in 14 cases of BLEL were both zero percent. (2) LMP1 expression was detected in 77.8% (14/18) of MLEL, EBNA2 was examined in 9 cases of MLEL and no expression was found. (3) Among the infiltrating lymphocytes studied by immunohistochemistry, T cells predominated over B cells in MLEL, B cells predominated over T cells in BLEL. (4) Of the 216 cases of salivary gland cancer, 37 cases could satisfy the criteria for MLEL. The incidence of MLEL in this group was 17.13% (37/216). Conclusion Our results suggest that EBV infection may have some relationship with the genesis of MLEL.